LOCUS       sequence-52329-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Ito-Harashima S, McCusker JH
  TITLE     Positive and negative selection LYS5MX gene replacement cassettes 
            for use in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2004 Jan 15;21(1):53-61.
  PUBMED    14745782
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2004-01-15"; volume: "21(1)"; pages: "53-61"
COMMENT     Sequence Label: pSA53
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..58)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(121..318)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cactagtgga tctgatatca cctaataact tcgtatagca tacattatac gaagttatat
       61 taagggttct cgagagctcg ttttcgacac tggatggcgg cgttagtatc gaatcgacag
      121 cagtatagcg accagcattc acatacgatt gacgcatgat attactttct gcgcacttaa
      181 cttcgcatct gggcagatga tgtcgaggcg aaaaaaaata taaatcacgc taacatttga
      241 ttaaaataga acaactacaa tataaaaaaa ctatacaaat gacaagttct tgaaaacaag
      301 aatcttttta ttgtcagtac tgatcactgg tttaaatgtt cagataataa tttttcaaat
      361 ctcaactcta ttggttgaat tgatttattc ttgttttgat taattatcgt tataattact
      421 ggcaatttag attcatcgac attaccatta tcaagaatag aactacgaca ataaaattct
      481 tttttattta atgccgttat aaattgattc ttggctaatt caagtttatc tatatttatc
      541 aagattttat caaaccaatt caatgaataa tacatccaac tatctccaat attatttcca
      601 actatacttg tttggaattt ttcattatca ctagaaaact cattatcatt ataatcaact
      661 atgaaataaa aatctgataa ttcaatattt aatccactac caatcaattt tgtaaatgat
      721 tcttttaatg tccaaaaatg attaaattta aaataattat caatttgtgg taattcataa
      781 acatcatcaa aaatcggttt aaattgttca agatattctg tatttgatat acta
//
