LOCUS       sequence-52395-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P41nmt1-GST
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             131..784
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     742..764
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 atcgaaacat tgaagataaa ggaagaggaa tcctggcata tcatcaattg aataagttga
       61 attaattatt tcaatctcat tctcactttc tgacttatag tcgctttgtt aaatcatgtc
      121 tttaattaat atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
      181 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
      241 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
      301 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
      361 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
      421 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
      481 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
      541 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
      601 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
      661 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
      721 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
      781 aaaatcggat ctggttccgc gttgaggcgc gccagatccg tcgacctgca gcgtacgaag
      841 cttcagctgg cggccgcgtt ctata
//
