LOCUS       sequence-52409-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ22m
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    123..139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    171..192
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      195..238
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(242..260)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        328..432
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(651..670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 attcaacaag aattgggaca actccagtga aaagttcttc tcctttacgg ctagtcatta
       61 aacttatctc ctctttaacg tgtgggccaa agaagaaaca agttgacggc gtgctcagta
      121 tcttgttatc cgctcacaat gtcaatgtta tccgctcaca tttatgctag ctctagaagc
      181 ggccgcgaat tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc
      241 tgcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      301 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      361 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      421 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      481 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      541 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      601 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      661 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      721 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      781 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      841 ctgacaacga tcggaggacc gaa
//
