LOCUS       sequence-52521-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Lungu OI, Hallett RA, Choi EJ, Aiken MJ, Hahn KM, Kuhlman B
  TITLE     Designing photoswitchable peptides using the AsLOV2 domain.
  JOURNAL   Chem Biol. 2012 Apr 20;19(4):507-17.
  PUBMED    22520757
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-04-20"; volume: "19(4)"; pages: "507-17"
COMMENT     Sequence Label: pET21b-LOV-ipaA
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             532..549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(602..620)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      616..663
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(787..806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcatcatc atcatcatca tggcagcctg
       61 gcgaccaccc tggaacgtat tgaaaagaac tttgtgatta ccgatccgcg tctgccggat
      121 aacccgatta tttttgcgag cgatagcttt ctgcagctga ccgaatatag ccgtgaagaa
      181 attctgggcc gtaactgccg ttttctgcag ggcccggaaa ccgatcgtgc gaccgtgcgt
      241 aaaattcgtg atgcgattga taaccagacc gaagtgaccg tgcagctgat taactatacc
      301 aaaagcggca aaaaattttg gaacctgttt catctgcagc cgatgcgtga tcagaaaggc
      361 gatgtgcagt attttattgg cgtgcagctg gatggtaccg aacatgtgcg tgatgcggcg
      421 gaacgtgaag gcgtgatgct gatcaagaaa accgcgaaca acattattaa agcggcgaaa
      481 gatgtgacca ccagcctgag caaagtgctg aaaaacatta actaactcga gcaccaccac
      541 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      601 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      661 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      721 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      781 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      841 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacg
//
