LOCUS       sequence-52559-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Kaykas A, Yang-Snyder J, Heroux M, Shah KV, Bouvier M, Moon RT
  TITLE     Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type 
            Frizzled in the endoplasmic reticulum by oligomerization.
  JOURNAL   Nat Cell Biol. 2004 Jan . 6(1):52-8.
  PUBMED    14688793
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2004 Jan . 6(1):52-8."
COMMENT     Sequence Label: XE240 Fz1-ER-GFP
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..766)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(98..119)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     447..466
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     706..727
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tacgactcac tatagttcta gactcgagtc agacggtagt ctccccttgt ttgctcttgt
       61 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      121 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
      181 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      241 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
      301 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
      361 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
      421 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
      481 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
      541 ggtcggggta gcggctgaag cactgcacgc cgtagctcag ggtggtcacg agggtgggcc
      601 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
      661 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
      721 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgct
//
