LOCUS       sequence-52681-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00489
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(579..678)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(695..714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(696..714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(719..735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(719..735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg caccatgtcg gcacgcaagg agaaacgaga
      121 catacattac tggcgaaaac aggcagccac cttgccggat tttgtgccgc cgtggaagcg
      181 ggaacaggat ttgcagcagc tgcaacatcg agcctcgatc ctgtggtcgc cgcaactgca
      241 ggaccaagat gacaaggctg ttcgcgaata ccttgactat gcggccagtc tctatgatat
      301 cgaggaggag caggcgctgt tcatcctgcg gcgtcacgga tacgacctgc ccctggcaca
      361 tcgacgtctg gagaagactg agacagctcg aggatgtcgc taccatcgct ggaaggccga
      421 ggatctcatc cgtcttacca aggccttcga gcaatacggc accgacttcg caaaggtacg
      481 aaaggagcta ccccactttc ccctggccga gcttcggctg tacttctcct tcatgtcctc
      541 ggcgttggag acagatgacc aacaggccat ccatagattg ccaactttct tgtacaaagt
      601 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
      661 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
      721 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acat
//
