LOCUS       sequence-52691-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MDH2
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..108
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac atatgcacca tcatcatcat
       61 cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat gtcggcccag
      121 aacaatgcta aagtagctgt gctaggggcc tctggaggca tcgggcagcc actttcactt
      181 ctcctgaaga acagcccctt ggtgagccgc ctgaccctct atgatatcgc gcacacaccc
      241 ggagtggccg cagatctgag ccacatcgag accaaagccg ctgtgaaagg ctacctcgga
      301 cctgaacagc tgcctgactg cctgaaaggt tgtgatgtgg tagttattcc ggctggagtc
      361 cccagaaagc caggcatgac ccgggacgac ctgttcaaca ccaatgccac gattgtggcc
      421 accctgaccg ctgcctgtgc ccagcactgc ccggaagcca tgatctgcgt cattgccaat
      481 ccggttaatt ccaccatccc catcacagca gaagttttca agaagcatgg agtgtacaac
      541 cccaacaaaa tcttcggcgt gacgaccctg gacatcgtca gagccaacac ctttgttgca
      601 gagctgaagg gtttggatcc agctcgagtc aacgtccctg tcattggtgg ccatgctggg
      661 aagaccatca tccccctgat ctctcagtgc acccccaagg tggactttcc ccaggaccag
      721 ctgacagcac tcactgggcg gatccaggag gccggcacgg aggtggtcaa ggctaaagcc
      781 ggagcaggct ctgccaccct ctccatggcg tatgccggcg cccgctttgt cttctccctt
      841 gtggatgcaa tgaatggaaa ggaaggtgtt gtggaatgtt ccttcgttaa gtcacaggaa
      901 acggaatgta cctacttctc cacaccgctg
//
