LOCUS       sequence-52709-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-QN
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(196..220)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(272..298)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(278..304)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(341..406)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(416..434)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(477..496)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          479..612
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ggcctttccc gaccgttcga ttgtaatcac caatatttag gctatgttta tctatctcca
       61 ttcgtttgtc ttgtaacatt cctccagaaa ggtccatctt attgctttgt acattttcct
      121 ctggtgagtc tctcgaaaaa ctgatgtttg aaaactgttt aacaaatgga tttatccacg
      181 cttcttcttg tttgaagcct gcttttttgt acaaacttgt tgatgggtcg actctagagg
      241 taccacgcgt gaattcgacg ggatccgcgt cgagtgaagc gtaatctgga acatcatatg
      301 ggtaggccat ggtgtaagct tgggcagcct cgagcggtgg gtttgcagct ttccattgag
      361 cttgtttctc agcgcgacgc tcacgtcgtc gtgtttgtgc gtccatggtg gctagcctat
      421 agtgagtcgt attaagtact ctagccttaa gagctgtaat tgaactggga gtggacacct
      481 gtggagagaa aggcaaagtg gatgtcagta agaccaatag gtgcctatca gaaacgtcga
      541 gagtcttctc tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac
      601 cttgatactt acctgcccag tgcctcacga ccaacttctg caaggctacc ttaagagagt
      661 cactgcagct taagttcgag actgttgtgt cagaagcact gactgcgtta gcaatttaac
      721 tgtgataaac taccgcaata aagcttctag tgatctgacg
//
