LOCUS       sequence-52716-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAP3K5
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg
       61 tagatctggg taccgagaac ctgtacttcc aatccatgag aagcacagag gaaggagact
      121 gtgaaagtga cttgctggag tatgactatg aatatgatga aaatggtgac agagtcgttt
      181 taggaaaagg cacttatggg atagtctacg caggtcggga cttgagcaac caagtcagaa
      241 ttgctattaa ggaaatccca gagagagaca gcagatactc tcagcccctg catgaagaaa
      301 tagcattgca taaacacctg aagcacaaaa atattgtcca gtatctgggc tctttcagtg
      361 agaatggttt cattaaaatc ttcatggagc aggtccctgg aggaagtctt tctgctctcc
      421 ttcgttccaa atggggtcca ttaaaagaca atgagcaaac aattggcttt tatacaaagc
      481 aaatactgga aggattaaaa tatctccatg acaatcagat agttcaccgg gacataaagg
      541 gtgacaatgt gttgattaat acctacagtg gtgttctcaa gatctctgac ttcggaacat
      601 caaagaggct tgctggcata aacccctgta ctgaaacttt tactggtacc ctccagtata
      661 tggcaccaga aataatagat aaag
//
