LOCUS       sequence-52739-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AKR1C4
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cccctctaga ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggatccc
      121 aaatatcagc gtgtagagct aaatgatggt cacttcatgc ccgtattggg atttggcacc
      181 tatgcacctc cagaggttcc gaggaacaga gctgtagagg tcaccaaatt agcaatagaa
      241 gctggcttcc gccatattga ttctgcttat ttatacaata atgaggagca ggttggactg
      301 gccatccgaa gcaagattgc agatggcagt gtgaagagag aagacatatt ctacacttca
      361 aagctttggt gcactttctt tcaaccacag atggtccaac cagccttgga aagctcactg
      421 aaaaaacttc aactggacta tgttgacctc tatcttcttc atttcccaat ggctctcaag
      481 ccaggtgaga cgccactacc aaaagatgaa aatggaaaag taatattcga cacagtggat
      541 ctctctgcca catgggaggt catggagaag tgtaaggatg caggattggc caagtccatc
      601 ggggtgtcaa acttcaacta caggcagctg gagatgatcc tcaacaagcc aggactcaag
      661 tacaagcctg tctgcaacca ggtagaatgt catccttacc tcaaccagag caa
//
