LOCUS       sequence-52781-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CUL5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(98..121)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(122..139)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(140..160)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcactt atcgtcgtca tccttgtaat
      121 catggtggtg atgatggtgc gattggaagt agaggttctc tgctttaaat atagtagcat
      181 cattaacaac tgctttatat gccttatctc ttgcagtaag gaaacgagga tcatcctgaa
      241 aagcttcttt gaccagttta ctgaatctat taaacagtgt gtctaattgc tcccgatact
      301 tctcagagtc agtagtgatg gtttcagctg cggccaccat gtctgctagg cccgcactta
      361 taatatgctc ctccaagtcc ttcaacatcg gctctatccc attaggaact ttgtccatca
      421 aggaaaacat caaatgtaac ttttcagttt catttcgctt gatcatgcct tggcattctg
      481 ctaaaatagt ctctttaaag gaggtcacca gcgcatttac acagcattcc atgagtgctt
      541 ccacagagtt acactctcgt cgtgtttcta agtatcggag tgctcttttt tcttcttctt
      601 ttaatttagc atctgcatat ttcatgtaat tctgcacacc attttgctgt aaatatgagg
      661 gtgcctgtgt tctataaaac ctctctgttg agtccaagta tgccttctca aaattatccc
      721 tatagatctg aagcttgtcc tcggggttgg agcaaagatt aacataggac tctcgcaccc
      781 cgatgaccag ctgggaatca aaagcttccc ctaatctctc agcatgcacc agcttcattg
      841 cactgtcctg gagtctgttc tttatatttg aaaaaatcga ctcattccac gtatcaagca
      901 tgagctttcg aacaatactg tcttccatat ttgatttttt attgctgctt tgtttaccca
      961 atagagtcac ctctaattga caaaaaggtt ttgg
//
