LOCUS       sequence-52797-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: VRK2
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..206
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
      181 gtaccgagaa cctgtacttc caatccatgc catttccaga aggcaaggtt ctggatgata
      241 tggaaggcaa tcagtgggta ctgggcaaga agattggctc tggaggattt ggattgatat
      301 atttagcttt ccccacaaat aaaccagaga aagatgcaag acatgtagta aaagtggaat
      361 atcaagaaaa tggcccgtta ttttcagaac ttaaatttta tcagagagtt gcaaaaaaag
      421 actgtatcaa aaagtggata gaacgcaaac aacttgatta tttaggaatt cctctgtttt
      481 atggatctgg tctgaccgaa ttcaagggaa gaagttacag atttatggta atggaaagac
      541 taggaataga tttacagaag atctcaggcc agaatggtac ctttaaaaag tcaactgtcc
      601 tgcaattagg tatccgaatg ttggatgtac tggaatatat acatgaaaat gaatatgttc
      661 atggtgatat aaaagcagca aatctacttt tgggttacaa aaatccagac caggtttatc
      721 ttgcagatta tggactttcc tacagatatt gtcccaatgg gaaccacaaa cagtatc
//
