LOCUS       sequence-52892-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CA6
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             850..870
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             871..888
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             889..912
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aatattttgt ttaccttaag aaggagatat actatgtctg actgcaccta ctcagaaggg
       61 gcactggacg aagcgcactg gccacagcac taccccgcct gtgggggcca gagacagtcg
      121 cctatcaacc tacagaggac gaaggtgcgg tacaacccct ccttgaaggg gctcaatatg
      181 acaggctatg agacccaggc aggggagttc cccatggtca acaatggcca cacagtgcag
      241 atcagcctgc cctccaccat gcgcatgaca gtggctgacg gcactgtata catagcccag
      301 cagatgcact ttcactgggg aggtgcgtcc tcggagatca gcggctctga gcacaccgtg
      361 gacgggatca gacatgtgat cgagattcac attgttcact acaattctaa atacaagagc
      421 tatgatatag cccaagatgc gccggatggt ttggctgtac tggcagcctt cgttgaggtg
      481 aagaattacc ctgaaaacac ttattacagc aacttcattt ctcatctggc caacatcaag
      541 tacccaggac aaagaacaac cctgactggc cttgacgttc aggacatgct gcccaggaac
      601 ctccagcact actacaccta ccatggctca ctcaccacgc ctccctgcac tgagaacgtc
      661 cactggtttg tgctggcaga ttttgtcaag ctctccagga cacaggtttg gaagctggag
      721 aattccttac tggatcaccg caacaagacc atccacaacg attaccgcag gacccagccc
      781 ctgaaccaca gagtggtgga atccaacttc ccgaatcagg aatacactct aggctctgaa
      841 ttccaggcag agaacctcta cttccaatcg caccatcatc accaccatga ttacaaggat
      901 gacgacgata agtgaggatc ccgaattcga ctcgtcaca
//
