LOCUS       sequence-52911-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MAGEA4
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             822..839
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggacgc agagtccttg
      121 ttccgagaag cactcagtaa caaggtggat gagttggctc attttctgct ccgcaagtat
      181 cgagccaagg agctggtcac aaaggcagaa atgctggaga gagtcatcaa aaattacaag
      241 cgctgctttc ctgtgatctt cggcaaagcc tccgagtccc tgaagatgat ctttggcatt
      301 gacgtgaagg aagtggaccc caccagcaac acctacaccc ttgtcacctg cctgggcctt
      361 tcctatgatg gcctgctggg taataatcag atctttccca agacaggcct tctgataatc
      421 gtcctgggca caattgcaat ggagggcgac agcgcctctg aggaggaaat ctgggaggag
      481 ctgggtgtga tgggggtgta tgatgggagg gagcacactg tctatgggga gcccaggaaa
      541 ctgctcaccc aagattgggt gcaggaaaac tacctggagt accggcaggt acccggcagt
      601 aatcctgcgc gctatgagtt cctgtggggt ccaagggctc tggctgaaac cagctatgtg
      661 aaagtcctgg agcatgtggt cagggtcaat gcaagagttc gcattgccta cccatccctg
      721 cgtgaagcag ctttgttaga ggaggaagag ggagtctgac agtaaaggtg gatacggatc
      781 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      841 ga
//
