LOCUS       sequence-53034-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             581..598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(651..669)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      665..712
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gagatataca tatgcaccat catcatcatc attcttctgg tgtagatctg ggtaccgaga
       61 acctgtactt ccaatccatg aacgttaccc tgctgatcca ggaactgatc cacaatctgt
      121 tcgtgagcgt gatgagccac caggatgatg aaggtcgctg ctatagcgat agcctggcgg
      181 aaatcccggc ggttgatccg aattttccga acaaaccgcc gctgaccttt gatatcattc
      241 gcaaaaacgt tgaaaacaac cgttatcgtc gtctggatct gtttcaggaa cacatgtttg
      301 aagtgctgga acgtgcgcgt cgtatgaacc gtaccgatag cgaaatctat gaagatgcgg
      361 tggaactgca gcagttcttt attaaaattc gcgatgaact gtgcaaaaat ggtgaaatcc
      421 tgctgagccc ggccctgagc tataccacca aacatctgca taatgatgtt gaaaaagaac
      481 gtaaagaaaa actgccgaaa gaaattgaag aagattgaca gtaaaggtgg atacggatcc
      541 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      601 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      661 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      721 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      781 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      841 ttcgcttttc ttccccttcc ctttctcgcc acgttcgccg gctttt
//
