LOCUS       sequence-53083-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AKT3
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             97..117
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             537..554
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(607..625)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      621..668
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(792..811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tggggtaatt ccctctgaat attttgttta ctttaagaag gagatataca tatgcaccat
       61 catcatcatc attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccatg
      121 agcgatgtta ccattgtgaa agaaggttgg gttcagaaga ggggagaata tataaaaaac
      181 tggaggccaa gatacttcct tttgaagaca gatggctcat tcataggata taaagagaaa
      241 cctcaagatg tggatttacc ttatcccctc aacaactttt cagtggcaaa atgccagtta
      301 atgaaaacag aacgaccaaa gccaaacaca tttataatca gatgtctcca gtggactact
      361 gttatagaga gaacatttca tgtagatact ccagaggaaa gggaagaatg gacagaagct
      421 atccaggctg tagcagacag actgcagagg caagaagagg agagaatgaa ttgacagtaa
      481 aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
      541 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      601 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      661 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      721 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      781 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      841 ccgtcaagct ctaaatcggg ggctcccttt agggt
//
