LOCUS       sequence-53086-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD1A
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..80
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             550..567
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaggagatat acatatgcac catcatcatc atcattcttc tggtgtagat ctgggtaccg
       61 agaacctgta cttccaatcc atggcggcgc ccccgcagcc cgtcacccac ctcatctttg
      121 acatggacgg acttcttctg gatactgaac ggctgtattc agtggtgttt caagaaatat
      181 gtaatcgcta tgacaagaaa tacagctggg atgtaaagtc cctggttatg ggtaagaagg
      241 cattagaggc ggcacagatt ataatagacg tcttgcagct cccgatgtcc aaagaggagc
      301 tggtggaaga aagccaaacg aagttaaagg aagtgttccc cacggctgcg ctcatgccag
      361 gggcggagaa actcatcatc cacctgcgga aacatggcat cccctttgca ctggccacca
      421 gctcgaggtc cgcgtcgttc gatatgaaga caagccgcca caaggagttc ttcagcttgt
      481 tttcccacat tgtgctggga gatgaccccg aagtgcagca tggcaagcca gacccagaca
      541 tcttcctagc ttgtgccaag aggttctctc cccctcctgc tatggagaag tgccttgtct
      601 ttgaagatgc tcccaatggg gtggaggcgg ccctggcagc tgggatgcag gtggtcatgg
      661 ttcctgacgg aaacttgagc cgagatctga caacaaaggc caccctggtg ctgaattccc
      721 tgcaggactt ccagcccgag ctgtttggtt tgccctccta tgagtgacag taaaggtgga
      781 tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
      841 accaccactg aggatcc
//
