LOCUS       sequence-53254-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: TTC5
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..81
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             642..659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaggagata tacatatgca ccatcatcat catcattctt ctggtgtaga tctgggtacc
       61 gagaacctgt acttccaatc catgcggcaa cgagagcaac aacttctgga attcctggat
      121 agattaacca gcctccttga gagtaaggga aaggtgaaga ccaaaaagct gcagagcatg
      181 ctgggaagct tgcgcccagc ccatctaggc ccttgcagtg atgggcacta tcagtcagcc
      241 tctgggcaga aagtgaccct ggagctcaag ccactgagta cgcttcagcc tggggtgaac
      301 agcggtgccg tcatcctggg aaaggtggta tttagcctca ccacagagga gaaagtcccc
      361 tttacatttg gcctggtaga ttcagatgga ccttgctatg cagtgatggt gtacaatata
      421 gtgcagagct ggggagtgct cattggagac tctgtagcca ttcctgagcc caacctgcgg
      481 cttcaccgaa ttcagcacaa aggaaaggac tattcctttt ccagtgttcg agtggagacg
      541 cccctcctgc tagtggtgaa tgggaagcct cagggatgac agtaaaggtg gatacggatc
      601 cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact
      661 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
//
