LOCUS       sequence-53264-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: VRK1
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcgtgt aaaagcagct
      121 caagctggaa gacagagctc tgcaaagaga catcttgcag aacaatttgc agttggagag
      181 ataataactg acatggcagc agcggcatgg aaagtaggat tacccattgg ccaaggaggc
      241 tttggctgta tatatcttgc tgatatgaat tcttcagagt cagttggcag tgatgcacct
      301 tgtgttgtaa aagtggaacc aagtgacaat ggacctcttt ttactgaatt aaagttctac
      361 caacgagctg caaaaccaga gcaaattcag aaatggattc gtacccgtaa gctgaagtac
      421 ctgggtgttc ctaagtattg ggggtctggt ctacatgaca aaaatggaaa aagttacagg
      481 tttatgataa tggatcgctt tgggagtgac cttcagaaaa tatatgaagc aaatgccaaa
      541 aggttttctc ggaaaactgt cttgcagcta agcttaagaa ttctggatat tctggaatat
      601 attcacgagc atgagtatgt gcatggagat atcaaggcct caaatcttct tctgaactac
      661 aagaatcctg accaggtgta cttggtagat tatggccttg cttatcggta ctgcccagaa
      721 ggagttcata aagcatacgc agcagacccc aaaagatgtc acgatggcac tattgaattc
      781 acgagcatcg atgcacacaa tggcgtggcc ccatcaagac gtggtgattt ggaaatactt
      841 ggttattgca tgatccaatg gcttactggc catcttcctt gggaggataa tttgaaagat
      901 cctaaatatg ttagagattc caaa
//
