LOCUS       sequence-53354-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of humanized ChR2 with H134R mutation fused to 
            EYFP for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Apr 5. 446(7136):633-9."
COMMENT     Sequence Label: pcDNA3.1/hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..238
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      280..289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
       61 agcgggcgaa gcactgcagg ccgtagccga aggtggtcac gagggtgggc cagggcacgg
      121 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      181 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      241 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg gccgctggca
      301 cggctccggc ctcggcttcg tcttcgacga gagtctcgac ctcgatctcc gttccgccga
      361 tgttcagttt ggtggttttg cggatatctc cgtgaatcaa tatgtgctcg tggatcagga
      421 cgcgcaggta gtgtcccaac aacccccaac aatttttact catcagatca ataatcgtgt
      481 gacctacggt ggagccatag acgctcagga cgccaaaacc ttcgggcccc aaaatgaaga
      541 gaattgggaa cataccccag ctcacgaaaa acagccatgc catgccggtc acgacctggc
      601 ggcaccgacc ctttggcaca gtatgataac cctcgatata tgctttggcg gcgtgaaaaa
      661 atgtgttcgc gccatagcac aatccaagac aaaagaagat gactttaaca tagccggttg
      721 ccatggcgct ggtagccccc cacacgatag tcccgatgtc tgagacaagg agtcccatgg
      781 ttctcctgct gtagtcgttg ctcaggccgg tgaggttgct caggcggata a
//
