LOCUS       sequence-53374-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MACROD1
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      824..833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
       61 cgacggagct cgaattcgga tccgtatcca cctttactgt caggccacgg ggaagtagtg
      121 ggggagccgg ctccggtaga tgtcctcgtc cttctcgagg aacacgcaga tgatcagccg
      181 gtccaccttg tccttgtgct gctccagcca ctctcgcagc gtggccagca cgatctcggc
      241 ggccgcctca caggggtagc caaacacgcc ggtggagatg caggggaacg ccaccgagcg
      301 gagccggtgc tccagcagca ggtccagact gctcaggtag cagctgcgga gctcggcagc
      361 ctggctggcg ctgggctccc cgtaggcgat gggccccact gtgtggatga cgtacttggc
      421 cgggagccga tagccgccgg tgatcttggc cttgccagtc ttacagctct gcagggtccg
      481 gcactcgtcg gtaagcaggg ggccggcggc ccgatgaatg cagccgtcca cgccaccgcc
      541 tccgagcagg gagctgttgg cggcgttgac gatggcgtcc acctccagct tggtgatgtc
      601 gctgcggagc agggagattt tctcattgag ctgcttgtcc tttttatacc tgggctcctc
      661 caccttcaca gccacccctt tcgccatctc cttccatgtc gggatcttct tcagcctgac
      721 aaagtccttg cagaagtaat gttcctcccg ctgcttgtca ctcaggccct tcagaaagga
      781 tttcgcctcc ttccagtcgg tggaggtgct caggtccacc ttcgccgcca tggccagggg
      841 gg
//
