LOCUS       sequence-53403-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P110alpha
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(216..240)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             293..334
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(371..391)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        376..705
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      556..691
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     618..637
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        717..764
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gaactgcaat cttttgatga tattgcatac attcgaaaga ccctagcttt agataaaact
       61 gagcaagagg ctttggagta tttcatgaaa caaatgaatg atgcacacca tggtggctgg
      121 acaacaaaaa tggattggat cttccacaca attaagcagc atgctttgaa cctcgagatg
      181 gcctacccct acgacgtgcc cgactacgcc tcctgaccca actttcttgt acaaagtggt
      241 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
      301 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttggaa
      361 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      421 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      541 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
      661 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccctgttg
      721 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
      781 ccatggccaa gttgaccagt gccgttccg
//
