LOCUS       sequence-53408-e        1791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Azpiazu I, Gautam N
  TITLE     A fluorescence resonance energy transfer-based sensor indicates that
            receptor access to a G protein is unrestricted in a living mammalian
            cell.
  JOURNAL   J Biol Chem. 2004 Jun 25;279(26):27709-18. Epub 2004 Apr 12.
  PUBMED    15078878
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-06-25"; volume: "279(26)"; pages: "27709-18. 
            Epub 2004 Apr 12"
COMMENT     Sequence Label: alpha 0-CFP in pdest12.2
FEATURES             Location/Qualifiers
     source          1..1791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             283..996
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPKEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(313..341)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     936..956
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgggatgta ctctgagcgc agaggagaga gccgccctcg agcggagcaa ggcgattgag
       61 aaaaatctca aagaagatgg catcagcgcc gccaaagacg tgaaattact cctgctgggg
      121 gctggagaat caggaaaaag caccattgtg aagcagatga agatcatcca tgaagatggc
      181 ttctctggag aagacgtaaa gcagtacaag cctgtcgtct acagcaacac catccagtct
      241 ctggcagcca ttgtgcgggc catggatact ctgggcggta ccatgagtaa aggagaagaa
      301 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      361 ttttctgtga gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      421 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tctcacttgg
      481 ggtgttcaat gcttttcaag atacccagat catatgaaac agcatgactt tttcaagagt
      541 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      601 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      661 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactatatc
      721 tcacacaatg tatacatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa
      781 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      841 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      901 ctttcgaaag atcccaaaga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      961 gctgggatta cacatggcat ggatgaacta tacaaaacta gtgtggagta tggtgacaag
     1021 gagaggaagg cagactccaa gatggtgtgt gacgtggtga gtcgcatgga ggacactgaa
     1081 ccattctctg cagaactgct ttctgccatg atgcgactct ggggcgactc ggggatccag
     1141 gagtgcttca accgatctcg ggagtatcag ctcaacgact ctgccaaata ctacctggac
     1201 agcttggatc ggattggagc cgctgactac cagcccaccg agcaggacat cctccgaacc
     1261 agggtcaaaa caactggcat cgtagaaacc cacttcacct tcaagaacct ccacttcagg
     1321 ctgtttgacg ttgggggcca gcgatctgaa cgtaagaagt ggatccactg cttcgaggat
     1381 gtcacggcca tcatcttctg tgtcgcactc agcggctatg accaggtgct ccacgaggac
     1441 gaaaccacga accgcatgca cgagtctctc atgctcttcg actccatctg taacaacaag
     1501 tttttcatcg atacctccat cattctcttc ctcaacaaga aagacctctt tggcgagaag
     1561 attaagaagt cacccttgac catctgcttt cctgaatacc caggctccaa cacctatgaa
     1621 gacgcagctg cctacatcca aacacagttt gaaagcaaaa accgctcacc caacaaagaa
     1681 atttactgtc acatgacttg tgccacagac acgaataata tccaggtggt attcgacgcc
     1741 gtcaccgaca tcatcattgc caacaatctc cggggctgtg gcttgtactg a
//
