LOCUS       sequence-53426-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-MYEF2
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     49..73
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             173..196
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             200..223
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             224..250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    266..290
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
       61 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      121 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacca tgcaccacca
      181 ccaccaccac caccacatgg actacaagga cgacgatgac aagtaccctt atgacgtgcc
      241 cgattacgct ttgaaccaga tatcaacaag tttgtacaaa aaagcagaag ggccgtcaag
      301 gcccaccatg gcggacgcca acaaggccga ggtgcccggg gccactggtg gcgacagccc
      361 gcacctgcag cccgcagagc cgccgggcga gccgcggcga gagccgcacc ccgcggaggc
      421 ggagaagcag cagccgcagc acagcagcag ctccaatggc gttaaaatgg agaatgatga
      481 atcagcaaaa gaagagaaat ctgacttaaa ggaaaaatct acaggaagta agaaggccaa
      541 tagatttcat ccttattcaa aagacaagaa ttcgggcact ggagaaaaga agggtccaaa
      601 tcgtaacaga gttttcatta gcaacatccc atatgacatg aaatggcaag ctattaaaga
      661 tctaatgaga gagaaagttg gtgaggttac atacgtggag ctctttaagg atgcggaagg
      721 aaaatcaagg gg
//
