LOCUS       sequence-53473-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pDDGFP-A
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..118
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(122..130,131..145)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(294..312)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     562..581
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     756..779
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa ccatgggggg
       61 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
      121 ggatctgtac gacgatgacg ataaggatcc gagctcgaga atggcgagca agagcgagga
      181 ggtcatcaaa gagttcatgc gcttcaaggt gcgcttggag ggctccatga acggccacga
      241 gttcgagatc gagggcgagg gcgagggccg cccttacgag ggcacccaga ccgccaagct
      301 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc ccctgatcat
      361 gtacggctcc aagatgtacg tgaagcaccc cgccgacgtc cccgattaca tgaagctgtc
      421 cttccccgag ggcttcaagt gggagcgcgt gatgcacttc gaggacggcg gtctggtgac
      481 cgcgacacag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
      541 caccaacttc ccccccgacg gccccgtaat gcagaagaag accttgggct gggattatgc
      601 caccgagcgc ctgtaccccg aagaaggcgt gctgaagggc gagctcctgg ggcgcctgaa
      661 gctgaaggac ggcggcctca acctggtgga gtccaagacc atctacatgg ccaagaagcc
      721 cgtgcaactg cccggctact acttcgtgga caccaagctg gacatcacct cccacaacga
      781 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgggg
//
