LOCUS       sequence-53477-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Alford SC, Ding Y, Simmen T, Campbell RE
  TITLE     Dimerization-dependent green and yellow fluorescent proteins.
  JOURNAL   ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. 
            Epub 2012 Aug 14.
  PUBMED    23656278
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300050j";
            journalName: "ACS Synth Biol"; date: "2012-12-21- 21"; volume: "1"; 
            issue: "12"; pages: "569-75"
COMMENT     Sequence Label: pCalN-ddGFP-A
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtgccgcc accatggcct cgagaatgga agggaagtgg ttgctgtgta
      121 tgttactggt gcttggaact gctattgttg aggctcatga tggacatgat gatgatgtga
      181 ttgatattga ggatgacctt gacgatgtca ttgaagaggt agaagactca aaaccagata
      241 ccactgctcc tccttcatct cccaaggtta cttacaaagc tccagttcca acaggggaag
      301 tatattttgc tgattctttt gacagaggaa ctctgtcagg gtggatttta tccaaagcca
      361 agaaagacga taccgatgat gaaattgcca aatatgatgg aaagtgggag gtagaggaaa
      421 tgaaggagtc aaagcttcca ggtgataaag gacttgtgtt gatgtctcgg gccaagcatc
      481 atgccatctc tgctaaactg aacaagccct tcctgtttga caccaagcct ctcattgttc
      541 agtatgaggt taatttccaa aatggaatag aatgtggtgg tgcctatgtg aaactgcttt
      601 ctaaaacacc agaactcaac ctggatcagt tccatgacaa gaccccttat acgattatgt
      661 ttggtccaga taaatgtgga gaggactata aactgcactt catcttccga cacaaaaacc
      721 ccaaaacggg tatctatgaa gaaaaacatg ctaagaggcc agatgcagat ctgaagacct
      781 attttactga taagaaaaca catctttaca cactaatctt gaatccagat aatagttttg
      841 aaatactggt tgaccaatct gtggtg
//
