LOCUS       sequence-53488-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     HP0377
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28b(+)-HP0377 C92A
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             678..695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(748..766)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      762..810
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gtgccgcgcg gcagccatat gttagacgaa aatcttttaa
      121 gctctggctc tcaaagctcc aaagaattaa acgatgagcg agacaatata gacaaaaaga
      181 gttacgctgg tttagaagat gttttttcag acaataagtc cattagtcct aacgataaat
      241 acatgctttt agtttttggc cgtaatggtt gctcctatgc tgaaaggttt aaaaaagatc
      301 tcaaaaatgt caaagaattg cgcgactaca ttaaagagca ttttagcgct tactatgtca
      361 atatcagcta ctccaaagag catgatttta aagtcggcga taaaaataat gaaaaagaaa
      421 tcaaaatgtc cacagaagaa ttagcgcaaa tttatgccgt ccaatccacc cctacgattg
      481 ttttatccga taaaaccggc aaaaccatct atgaattgcc cggctatatg ccctctacgc
      541 aatttttagc cgtgttagaa tttatcggcg atgggaagta tcaagacaca aaagacgatg
      601 aggatctcac taaaaaatta aaggcttaca tcaagtataa aaccaacctt tctaaaagca
      661 agtctaacta actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      721 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa cccctttggg
      781 gcctctaaac gggtcttgag gggttttttg ctgaagggag gaactatatc cggattggcg
      841 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
//
