LOCUS       sequence-53515-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT
  TITLE     Chibby, a nuclear beta-catenin-associated antagonist of the 
            Wnt/Wingless pathway.
  JOURNAL   Nature. 2003 Apr 24. 422(6934):905-9.
  PUBMED    12712206
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 Apr 24. 422(6934):905-9."
COMMENT     Sequence Label: M48 GST-TEV-Chibby-N
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(306..328)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(473..491)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        559..663
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggttccgcg tggatccccg gaattcgaaa aacctgtatt ttcagggcat gcctttcttt
       61 gggaatacgt tcagtccgaa gaagacacct cctcggaagt cggcatctct ctccaacctg
      121 cattctttgg atcgatcaac ccgggaggtg gagctgggct tggaatacgg atccccgact
      181 atgaacctgg cagggcaaag cctgaagttt gaaaatggcc agtggatagc agagacatga
      241 ctcgagcggc cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt
      301 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
      361 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
      421 atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg
      481 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      541 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      601 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      661 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      721 cctgttttaa
//
