LOCUS       sequence-53592-e        1374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Conformation-Selective ATP-Competitive Inhibitors Control Regulatory
            Interactions and Noncatalytic Functions of Mitogen-Activated Protein
            Kinases.
  JOURNAL   Chem Biol. 2014 May 22;21(5):628-35. doi: 
            10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.
  PUBMED    24704509
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.02.016"; journalName: "Chem Biol"; date: 
            "2014-05-22- 22"; volume: "21"; issue: "5"; pages: "628-35"
COMMENT     Sequence Label: DUSP6
FEATURES             Location/Qualifiers
     source          1..1374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1324..1341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tnnnnctaga ntaattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc caatgccata
      121 gatacgctca gacccgtgcc cttcgcgtcg gaaatggcga tcagcaagac ggtggcgtgg
      181 ctcaacgagc agctggagct gggcaacgag cggctgctgc tgatggactg ccggccgcag
      241 gagctatacg agtcgtcgca catcgagtcg gccatcaacg tggccatccc gggcatcatg
      301 ctgcggcgcc tgcagaaggg taacctgccg gtgcgcgcgc tcttcacgcg cggcgaggac
      361 cgggaccgct tcacccggcg ctgtggcacc gacacagtgg tgctctacga cgagagcagc
      421 agcgactgga acgagaatac gggcggcgag tcggtgctcg ggctgctgct caagaagctc
      481 aaggacgagg gctgccgggc gttctacctg gaaggtggct tcagtaagtt ccaagccgag
      541 ttctccctgc attgcgagac caatctagac ggctcgtgta gcagcagctc gccgccgttg
      601 ccagtgctgg ggctcggggg cctgcggatc agctctgact cttcctcgga catcgagtct
      661 gaccttgacc gagaccccaa tagtgcaaca gactcggatg gtagtccgct gtccaacagc
      721 cagccttcct tcccagtgga gatcttgccc ttcctctact tgggctgtgc caaagactcc
      781 accaacttgg acgtgttgga ggaattcggc atcaagtaca tcttgaacgt cacccccaat
      841 ttgccgaatc tctttgagaa cgcaggagag tttaaataca agcaaatccc catctcggat
      901 cactggagcc aaaacctgtc ccagtttttc cctgaggcca tttctttcat agatgaagcc
      961 cggggcaaga actgtggtgt cttggtacat tgcttggctg gcattagccg ctcagtcact
     1021 gtgactgtgg cttaccttat gcagaagctc aatctgtcga tgaacgatgc ctatgacatt
     1081 gtcaaaatga aaaaatccaa catatcccct aacttcaact tcatgggtca gctgctggac
     1141 ttcgagagga cgctgggact cagcagccca tgtgacaaca gggttccagc acagcagctg
     1201 tattttacca ccccttccaa ccagaatgta taccaggtgg actctctgca atctacgtaa
     1261 cattggaagt ggataacgga tccgaattcg agctccgtcg acaaggttgc ggccgcactc
     1321 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaan gnng
//
