LOCUS       sequence-53593-e        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  TITLE     various new plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Pin1
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             662..679
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(732..750)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      746..793
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(919..938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cnnnnataan tttgnttaac tttaagaagg agatatacat atgcaccatc atcatcntcn
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccaatg cggacgagga
      121 gaagctgccg cccggctggg agaagcgcat gagccgcagc tcaggccgag tgtactactt
      181 caaccacatc actaacgcca gccagtggga gcggcccagc ggcaacagca gcagtggtgg
      241 caaaaacggg cagggggagc ctgccagggt ccgctgctcg cacctgctgg tgaagcacag
      301 ccagtcacgg cggccctcgt cctggcggca ggagaagatc acccggacca aggaggaggc
      361 cctggagctg atcaacggct acatccagaa gatcaagtcg ggagaggagg actttgagtc
      421 tctggcctca cagttcagcg actgcagctc agccaaggcc aggggagacc tgggtgcctt
      481 cagcagaggt cagatgcaga agccatttga agacgcctcg tttgcgctgc ggacggggga
      541 gatgagcggg cccgtgttca cggattccgg catccacatc atcctccgca ctgagtgaca
      601 ttggaagtgg ataacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
      661 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      721 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      781 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      841 agcggcgcat taagcgcggc ggggtgtggt ggttacgcgc agcgtgaccg ctacactttg
      901 ccagcgccct agcgcccgct cctttcgctt tcttcccttt cctttctcgn cacgttcgcc
      961 ggctttcccc gtnagctcta atcnggggct ccctttaggg ttccgattta ntgctttacg
     1021 cacctcgacc ccaaaaaact tgattagggn gannnncana ttaacgctta caatttaggt
     1081 ggcncttttn cnngggnaan ngtgcgnnnn nc
//
