LOCUS       sequence-53603-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Griffin EE, Odde DJ, Seydoux G
  TITLE     Regulation of the MEX-5 gradient by a spatially segregated 
            kinase/phosphatase cycle.
  JOURNAL   Cell. 2011 Sep 16;146(6):955-68.
  PUBMED    21925318
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-16"; volume: "146(6)"; pages: "955-68"
COMMENT     Sequence Label: pEG476
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             247..267
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             280..324
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 atggcactac tactagaagg tggaggtcac tacctttgcg attttaaaac tacttacaaa
       61 gcaaagaagg tcgtccaact tccagatgca cactttgttg atcacagaat tgaaatacta
      121 ggaaacgatt cggattacaa caaagttaag ctatacgaac acgcagttgc aagatacagt
      181 cctctaccaa gtcaagcatg gtgcccgggg gatcggtgga gctccaccgg tggcggccgc
      241 tcgagagaga atctttattt tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa
      301 ttcgaacgcc agcacatgga cagcggaggt ggaggtacta gtatgaaagc ggcatcaaat
      361 agtgtctcgt cggccggagg atcagtgtca cctacgacaa cccagccacc actaccacca
      421 gggcagtcgt cacatcctca aatctacgat caacagatgc agtactactt cgcggctgcc
      481 atgccgaacc agccaatggc gacgtatgcg gcacaaaatg gatcttctca acagtacgcc
      541 ccagcggctc catattatca agatgccaac ggacagtatg tgcaggttcc cgcgaacgga
      601 tcaatggctc ctcaacagca cat
//
