LOCUS       sequence-53646-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Schlorb C, Ackermann K, Richter C, Wirmer J, Schwalbe H
  TITLE     Heterologous expression of hen egg white lysozyme and resonance 
            assignment of tryptophan side chains in its non-native states.
  JOURNAL   J Biomol NMR. 2005 Oct;33(2):95-104.
  PUBMED    16258828
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomol 
            NMR."; date: "2005-10"; volume: "33(2)"; pages: "95-104"
COMMENT     Sequence Label: pET11a-4SS-HEWL
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(584..602)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      598..645
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgaaagtat ttggccgttg cgagcttgcg gcggcgatga
      181 aacgtcatgg cctggacaac taccgggggt attccttggg taactgggtt tgcgctgcga
      241 aattcgaaag caattttaat actcaagcca ccaatcgcaa caccgacggc tcgaccgatt
      301 atgggatctt acagattaat tctcgttggt ggtgtaacga cggtcgcaca ccaggtagta
      361 gaaatctctg caacatcccg tgtagcgccc tgctgtcatc ggatatcacg gcaagtgtca
      421 actgtgctaa gaaaattgtg agcgatggca atggtatgaa cgcatgggtt gcctggcgca
      481 accgctgtaa aggcacggat gtgcaggcat ggattcgagg ctgccgtctg taaggatccg
      541 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      601 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      661 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
      721 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
      781 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tc
//
