LOCUS       sequence-53772-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label:  pEMB018 LRRK2_BC_1520_2148_K1906M
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..102
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             103..120
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccatctcgca ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
       61 ataaatatag gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc
      121 ggacagctga tccccgactg ctacgtggaa cttgagaaga tcatcctgag cgagcgcaag
      181 aatgtgccta tcgagtttcc agtgatcgat cgcaaacgtc tcctccagct cgtgcgtgag
      241 aatcagctgc agctcgacga gaacgagctg cctcatgctg tgcacttcct taacgagtca
      301 ggtgtcctcc tgcacttcca ggacccggct ctccagctga gtgacctcta tttcgtggag
      361 ccaaagtggc tctgtaaaat tatggctcaa atcctcactg tcaaggtaga gggctgtcca
      421 aagcacccaa agggcatcat ctcgcgtcgc gacgtggaaa agtttctctc taagaagcgc
      481 aagtttccca agaactacat gagccagtac tttaaactgt tggagaagtt ccagatcgct
      541 ctgcctatcg gcgaagagta cctgttggtt ccttcctccc tgtccgatca ccgccccgtg
      601 attgagctgc ctcactgcga gaacagcgag attatcattc gcctatacga gatgccgtac
      661 tttccgatgg gcttctggtc aaggctcatc aatcgtctgc ttgaaatctc cccatatatg
      721 ctgagcggtc gtgaacgcgc tctgaggcca aaccggatgt actggcgcca ggggatttac
      781 ttgaactggt cccctgaggc ttattgtctg gtgggctcgg aagtcctgga taaccacccc
      841 gaatcattcc tgaagatcac cgttccttcc tgccgtaa
//
