LOCUS       sequence-53847-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Kim KH, An DR, Song J, Yoon JY, Kim HS, Yoon HJ, Im HN, Kim J, Kim 
            do J, Lee SJ, Kim KH, Lee HM, Kim HJ, Jo EK, Lee JY, Suh SW
  TITLE     Mycobacterium tuberculosis Eis protein initiates suppression of host
            immune responses by acetylation of DUSP16/MKP-7.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 15;109(20):7729-34. Epub 2012 Apr
            30.
  PUBMED    22547814
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-05-15"; volume: "109(20)"; pages: 
            "7729-34. Epub 2012 Apr 30"
COMMENT     Sequence Label: pET21a-MKP7_K62R_153
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             494..516
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    531..555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(555..574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(556..574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(658..677)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgaga ggtaagcaag gctgagaaat
       61 gcaggtaggg actagagtgg attttccttc acagaggcca gggaaacaac gagagaactc
      121 agcaaaccca cctgcaagca ggtgaacaga gttgaagctc ttctccagtc tacccagaag
      181 tacagtgaga aaacagtctg aagagagaga ggcaacatct tgggagcttt gatcgtaaac
      241 tacaaccttc tgactgcaat caatgtcaac cttatgtttc gctgaatgct ggatgagctc
      301 tgtaattaac acgcggtcct gttgcaacct tcgcttcata agcttggagc agttgatatt
      361 aatggcttcc aaaatgtggg atgtattgta ttccacaaat ggccggctat caattagcag
      421 cactttttcc gttccacttt ccagcagagc caccaacctc tcagtaacaa tttgagttcc
      481 aatcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      541 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc
      601 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      661 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      721 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      781 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      841 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgatgcgcaa
//
