LOCUS       sequence-53852-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     yeast Hbs1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28b(+)_S. cerevisiae Hbs1(162-611)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             178..195
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gccggcccga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg
      181 gtgccgcgcg gcagccatat ggctagcatg aaatctgcct tacctcactt aagttttgtt
      241 gttcttggtc atgttgatgc gggaaaatca actctaatgg gcagactact ttatgatctg
      301 aacattgtca accaatccca actgagaaag ctacaaagag agagtgaaac tatgggtaag
      361 tcatccttca aatttgcatg gattatggat cagacaaatg aagagcgcga acgtggtgta
      421 acagtgtcaa tttgtacatc gcatttctct acccataggg caaattttac tattgtggat
      481 gcgccaggcc atagagattt tgttccaaac gctataatgg ggatatcaca agcagatatg
      541 gctatacttt gtgtagactg tagcaccaat gctttcgaat caggatttga cttggatggg
      601 caaacaaagg agcatatgct actggcatct agtcttggaa ttcacaatct gattattgct
      661 atgaataaga tggataatgt tgactggtcc caacaaaggt ttgaagagat taaatcgaaa
      721 ttgttgccat atctggttga tattgggttt tttgaggata atattaattg ggtacctatc
      781 agtggctttt ccggagaagg tgtttataaa attgaatata cagatgaagt gagacaatgg
      841 tacaatggtc
//
