LOCUS       sequence-53860-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Chin Lab P85 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti4/V5-DEST-P85(D560Y)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(317..340)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(364..388)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    429..447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    450..468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(470..673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(499..519)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtttcgagac ctggtgcaca ggaagaggcc gaggtggccg gggctttggt gtgggaggcg
       61 agattttttt ccttccaata tattctacat aagttcccgg aaagtccccc ctttcccctg
      121 tggtttcatt atagccattt aaccagccaa tttcttcagg cctggcttcc tgtccatcac
      181 tgaatccaag agctactaag gaccctttat tcacagtcaa tatgtcaccc aagtgcaagt
      241 caatatcttc ttctctttcc tttttataat catacagcgc tctgtactgg tacccctcag
      301 cactcatggc catggatttg tcatcatcgt ccttatagtc catggtgaag ggggcggccg
      361 cggagcctgc ttttttgtac aaacttgttg atatctgcag aattccacca cactggacta
      421 gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga gctctgctta
      481 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      541 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      601 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
      661 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
      721 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
      781 caataggggg cgtactttgg
//
