LOCUS       sequence-53921-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB52/Horse_34_374
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..102
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             103..120
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catctcgcaa ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct
       61 ataaatatag gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc
      121 ggtgacctct tagttaaccc tgatcaacct cgtctgacta tcccgatctc ccaaatcgct
      181 cctgacctga ttctggctga tctccctcgt aacatcatgc tgaactcaga tgaactcgaa
      241 tttgaacagg ctccagagtt tctcttaggc gacggctctt tcggctctgt ttaccgtgca
      301 gcctacgagg gtgaagaagt agcagtgaaa atctttaaca aacacacttc cctccgcctg
      361 ctccgccagg aattagttgt gctgtgtcat ctgcaccacc cgtccctcat tagcctgctg
      421 gctgccggta tccgtcctcg catgctcgtt atggaattag ctagcaaggg cagcctggac
      481 cgtttgctgc agcaggataa agctagcctg acccgtaccc tgcagcaccg tattgccctt
      541 cacgtagctg atggtctccg ttacctgcat agcgccatga tcatctatcg tgacctcaag
      601 ccacataacg ttctgctgtt caccctctac ccgaacgcag ccataatcgc taagattgct
      661 gactacggca tcgctcaata ctgctgccgt atgggcatca agacttctga aggtactccg
      721 ggcttccgtg cgccagaagt agctcgcggc aacgtgattt ataaccagca ggctgacgtg
      781 tacagcttcg gcctcctgct ctacgacatt ctgaccaccg gcggccgcat agctgaaggc
      841 ctgaaattcc ctaacgaatt tgacgagctc gcaatccaag gcaagctccc agacccggta
      901 cgcgaatacg g
//
