LOCUS       sequence-53939-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1185_2136
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..90
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             91..108
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatatagga
       61 tctatgcacc accatcacca ccatcaccac ttggtgcccc gcggatcctt ctcgtgcatc
      121 ccagaggcaa tcctcaacct gcctcacctg cgtagcctgg acatgagttc caatgacatc
      181 cagtacctgc ccggtcctgc ccactggaag tcattgaatc tgcgtgaact cctgttctcc
      241 cataatcaga tttccatcct ggacctttca gagaaggcct acctctggtc tcgcgtggaa
      301 aagctacatc taagtcacaa caagctgaag gaaatccctc ctgagatcgg gtgcctggaa
      361 aacctgacct ccttggacgt ctcttataac ctggaactgc gttcctttcc taacgaaatg
      421 ggcaaactct ctaagatttg ggacctgcct ctggacgagc tgcacctaaa cttcgacttc
      481 aagcacattg gctgtaaagc aaaggatatt attcgtttcc tgcagcagcg tcttaagaag
      541 gccgtgccct acaatcgcat gaagctgatg atcgtcggga acaccggttc tggaaagaca
      601 accctcttgc agcagcttat gaagactaag aagtccgact tggggatgca gagcgccact
      661 gtggggatcg acgtgaaaga ctggccaatc caaatccgtg acaagcgcaa gcgtgactta
      721 gtactgaacg tgtgggactt tgccggcagg gaagagttct actctacgca tcctcacttc
      781 atgactcagc gtgccctcta tctggcagtt tacgacctct caaagggcca ggctga
//
