LOCUS       sequence-54054-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1300_2210
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             69..92
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             93..110
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataaataagt attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatatag
       61 gatctatgca ccaccatcac caccatcacc acttggtgcc ccgcggatcc gacgagctgc
      121 acctaaactt cgacttcaag cacattggct gtaaagcaaa ggatattatt cgtttcctgc
      181 agcagcgtct taagaaggcc gtgccctaca atcgcatgaa gctgatgatc gtcgggaaca
      241 ccggttctgg aaagacaacc ctcttgcagc agcttatgaa gactaagaag tccgacttgg
      301 ggatgcagag cgccactgtg gggatcgacg tgaaagactg gccaatccaa atccgtgaca
      361 agcgcaagcg tgacttagta ctgaacgtgt gggactttgc cggcagggaa gagttctact
      421 ctacgcatcc tcacttcatg actcagcgtg ccctctatct ggcagtttac gacctctcaa
      481 agggccaggc tgaggtggac gccatgaaac cctggctgtt taacatcaag gctcgtgctt
      541 ccagctcccc tgtgatctta gtcgggacac acttggatgt ttctgatgag aagcagcgta
      601 aggcatgtat gagtaagatc accaaagagc tgttgaacaa acgcggcttt cccgccatcc
      661 gtgattacca cttcgtcaac gctaccgagg aaagcgacgc actggccaag ttacgtaaga
      721 ccattatcaa cgaaagcctg aacttcaaaa ttcgcgacca gctggtggtc ggacagctga
      781 tccccgactg ctacgtggga
//
