LOCUS       sequence-54059-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1300_2148
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             75..98
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             99..116
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctcgcaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa
       61 atataggatc tatgcaccac catcaccacc atcaccactt ggtgccccgc ggatccgacg
      121 agctgcacct aaacttcgac ttcaagcaca ttggctgtaa agcaaaggat attattcgtt
      181 tcctgcagca gcgtcttaag aaggccgtgc cctacaatcg catgaagctg atgatcgtcg
      241 ggaacaccgg ttctggaaag acaaccctct tgcagcagct tatgaagact aagaagtccg
      301 acttggggat gcagagcgcc actgtgggga tcgacgtgaa agactggcca atccaaatcc
      361 gtgacaagcg caagcgtgac ttagtactga acgtgtggga ctttgccggc agggaagagt
      421 tctactctac gcatcctcac ttcatgactc agcgtgccct ctatctggca gtttacgacc
      481 tctcaaaggg ccaggctgag gtggacgcca tgaaaccctg gctgtttaac atcaaggctc
      541 gtgcttccag ctcccctgtg atcttagtcg ggacacactt ggatgtttct gatgagaagc
      601 agcgtaaggc atgtatgagt aagatcacca aagagctgtt gaacaaacgc ggctttcccg
      661 ccatccgtga ttaccacttc gtcaacgcta ccgaggaaag cgacgcactg gccaagttac
      721 gtaagaccat tatcaacgaa agcctgaact tcaaaattcg cgaccagctg gtggtcggac
      781 agctgatccc cgactgctac gtggaacttg agaagatcat cctgagcgag cgcaagaatg
      841 tgcctatcga gtttc
//
