LOCUS       sequence-54144-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRSII316
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(4..22)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    63..566
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        603..707
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
       61 cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg
      121 aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa
      181 gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa
      241 caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa atagatatac
      301 attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac
      361 agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta
      421 gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt
      481 ctttaatttc tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa
      541 ttataattat ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg
      601 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      781 cagaaacgct ggtgaa
//
