LOCUS       sequence-54181-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Chee MK, Haase SB
  TITLE     New and Redesigned pRS Plasmid Shuttle Vectors for Genetic 
            Manipulation of Saccharomycescerevisiae.
  JOURNAL   G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.
  PUBMED    22670222
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2012-05"; volume: "2(5)"; pages: "515-26. Epub 
            2012 May 1"
COMMENT     Sequence Label: pRS40P
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(52..249)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(303..755)
                     /codon_start=1
                     /gene="Streptomyces viridochromogenes pat1"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialaphos or phosphinothricin"
                     /translation="MAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVA
                     EVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSNLYTHLLKSMEAQGFKS
                     VVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDF"
ORIGIN
        1 attcgagctc gttttcgaca ctggatggcg gcgttagtat cgaatcgaca gcagtatagc
       61 gaccagcatt cacatacgat tgacgcatga tattaccttc tgcgcactta acttcgcatc
      121 tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attagaatag
      181 aacaactaca atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt
      241 attgtcagta ctgattagat ctgtgtgacg ggccggacgg ggcggagcgg ggccggcagc
      301 tcgaagtcgc gctgccagaa ccccacgtcg tgccagcccc cgtgcttgta gccggctgcc
      361 cgcagcgtcc cgcgcgcggt gtatccgagc gcctcgtgca ggcgcacgct cgggtcgttg
      421 ggcagtccga tgacggcgac cacgctcttg aagccctggg cctccatgga cttcagcagg
      481 tgggtgtaga ggttggagcc cagtccgagc cgctggtgcc ggtgggagac gtacaccgtc
      541 gactcgacgg tccagtcgta ggcgttgcgg gccttccagg ggccggcgta ggcgatgccg
      601 gcgacgacgc cctccacctc ggcgacgagc caggggtagc ggtcctggag gcgctccagg
      661 tcgtcgatcc actcctgcgg agtctgcggc tccgtacgga agttgaccgt gctcgtctcg
      721 atgtagtgat tgacgatgtc gcagaccgcc gccatgtcgg cggcggtggc gggacggatc
      781 tcgaccgg
//
