LOCUS       sequence-54200-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3-Camui-CR
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..719)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcccttact tgtacagctc gtccatccca ccaccaagcc cggcgaactt ggcaactgcg
       61 tgttcgcgtt gtactacgaa catttcattg tcactttcct ctaacctttc caggcggtga
      121 tcaacggcat ggataccggg catcttgatg ttcccgacgg tcttttttga cctgtaagtt
      181 gttacgaaag agcaagacag atggccacca ccatcaactt tcagtgccat atgagtgtat
      241 cccctcagac caccatctgc tggatacatc atctctgtat taggctccca acccttggtc
      301 ttcttctgca tcacgggacc attggaggga aagtttaccc ctctgacttg gacgtggtaa
      361 acgagacagc catcctcaag gctggtgtcc tgcatgacgg tgacgactcc accatcttcg
      421 tatctcgtaa ctctttccca agtaaaaccc tcaggaaagg actgtttaaa gaaatcagga
      481 atgcctttcg ggtacttgat aaaagtacgg ctgccataca tgaacgacgt ggcaagaatg
      541 tcaaaggcaa atggcagggg tcctccctcg atgactttga tcctcatggt ttgagttccc
      601 atgtacggat tgccttctcc ttcacctgtg catttgaatt ggtggccgtt gaccgaacct
      661 tccatgacca ccttcatacg catattttcc ttgatcagct cttcgccctt agacaccatg
      721 gtggcgaccg gtagcgctag tccatggggc aggacggagg gcgccccaga tctgtggaag
      781 tggacgatct gccattttcc atccctgcgg tgccagacac gggtctc
//
