LOCUS       sequence-54208-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: pcDNA3.1/Puro-CAG-VSFP-CR
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(83..793)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 agcggccgcc actgtgctgg atatctgcag aattccacca cactggacta gtggatccga
       61 gctcggtacc aagcttttat tacttgtaca gctcgtccat cccaccacca agcccggcga
      121 acttggcaac tgcgtgttcg cgttgtacta cgaacatttc attgtcactt tcctctaacc
      181 tttccaggcg gtgatcaacg gcatggatac cgggcatctt gatgttcccg acggtctttt
      241 ttgacctgta agttgttacg aaagagcaag acagatggcc accaccatca actttcagtg
      301 ccatatgagt gtatcccctc agaccaccat ctgctggata catcatctct gtattaggct
      361 cccaaccctt ggtcttcttc tgcatcacgg gaccattgga gggaaagttt acccctctga
      421 cttggacgtg gtaaacgaga cagccatcct caaggctggt gtcctgcatg acggtgacga
      481 ctccaccatc ttcgtatctc gtaactcttt cccaagtaaa accctcagga aaggactgtt
      541 taaagaaatc aggaatgcct ttcgggtact tgataaaagt acggctgcca tacatgaacg
      601 acgtggcaag aatgtcaaag gcaaatggca ggggtcctcc ctcgatgact ttgatcctca
      661 tggtttgagt tcccatgtac ggattgcctt ctccttcacc tgtgcatttg aattggtggc
      721 cgttgaccga accttccatg accaccttca tacgcatatt ttccttgatc agctcttcgc
      781 ccttagacac catctcgcct ccggactcga gggcggcggt tacgaactc
//
