LOCUS       sequence-54255-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Watton SJ, Downward J
  TITLE     Akt/PKB localisation and 3' phosphoinositide generation at sites of 
            epithelial cell-matrix and cell-cell interaction.
  JOURNAL   Curr Biol. 1999 Apr 22;9(8):433-6.
  PUBMED    10226029
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "1999-04-22"; volume: "9(8)"; pages: "433-6"
COMMENT     Sequence Label: pcDNA3-mAkt-ER
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(208..249)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      246..255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(303..322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(304..322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(367..570)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(396..416)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        571..950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggtgagtgtg tgggccacct cgtccttggc cacgatgact tccttcttga ggatcttcat
       61 ggcgtagtag cggcctgtgg ccttctcctt caccaggatc accttgccga aagtgccctt
      121 gcccagcagc ttcaggtact caaactcgtt catggtcacg cggtgcttgg gcttggccag
      181 ggacacctcc atctcttcag cctcgctgcg ctggctgggg tccttaggct tgctcttgct
      241 actccccatg gcggcgaatt ccccggggta ccggatccga gctcggtacc aagcttgggt
      301 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
      361 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      421 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      481 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      541 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      601 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      661 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      721 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      781 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      841 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      901 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aac
//
