LOCUS       sequence-54257-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pGTvL1-SGC
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..79)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        94..539
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 acctgtactt ccatccataa gctagcttct cctcctgaaa gatccataac ttcgtatagc
       61 atacattata cgaagttatg cggccgcgac gtccacatat acctgccgtt cactattatt
      121 tagtgaaatg agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc
      181 atgcaacaga aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc
      241 tttaggcccg tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag
      301 accagttgca atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt
      361 ttgttactga taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc
      421 gtcacccctt acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa
      481 acaggagggc tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga
      541 tgaacatcaa aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg
      601 caggaggcgc aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat
      661 acggcatttc ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg
      721 aaaaatataa agttcctgag ttcgattcgt ccacaattaa aaatatctct tctgcaaaag
      781 gcctggacgt ttgggacagc tggccattac aaaacactga cggcactgtc gcaaactatc
      841 acggctacca catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga
      901 tttacatgtt ctatcaa
//
