LOCUS       sequence-54283-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB018 / LRRK2_BC_1333_2232
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..99
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             100..117
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctcgcaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
       61 aatataggat ctatgcacca ccatcaccac catcaccact tggtgccccg cggatccaat
      121 cgcatgaagc tgatgatcgt cgggaacacc ggttctggaa agacaaccct cttgcagcag
      181 cttatgaaga ctaagaagtc cgacttgggg atgcagagcg ccactgtggg gatcgacgtg
      241 aaagactggc caatccaaat ccgtgacaag cgcaagcgtg acttagtact gaacgtgtgg
      301 gactttgccg gcagggaaga gttctactct acgcatcctc acttcatgac tcagcgtgcc
      361 ctctatctgg cagtttacga cctctcaaag ggccaggctg aggtggacgc catgaaaccc
      421 tggctgttta acatcaaggc tcgtgcttcc agctcccctg tgatcttagt cgggacacac
      481 ttggatgttt ctgatgagaa gcagcgtaag gcatgtatga gtaagatcac caaagagctg
      541 ttgaacaaac gcggctttcc cgccatccgt gattaccact tcgtcaacgc taccgaggaa
      601 agcgacgcac tggccaagtt acgtaagacc attatcaacg aaagcctgaa cttcaaaatt
      661 cgcgaccagc tggtggtcgg acagctgatc cccgactgct acgtggaact tgagaagatc
      721 atcctgagcg agcgcaagaa tgtgcctatc gagtttccag tgatcgatcg caaacgtctc
      781 ctccagctcg tgcgtgagaa tcagctgcag ctcgacgaga acgagctgcc tcatgctgtg
      841 cacttcttac
//
