LOCUS       sequence-54297-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEMB12 / LRRK2_BC_1847_2210
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             419..436
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct agcaggagga
       61 attcatgaag catcaccacc atcaccatct cgagtccgat aagataatcc acctgactga
      121 cgactccttc gataccgacg tgctgaaggc agacggtgct atcctcgttg acttctgggc
      181 ccactggtgt ggtccttgca agatgatagc tcctattctt gacgagatcg cagacgaata
      241 ccaaggtaag ctgaccgttg ctaagctgaa cattgatcat aaccctggca ccgcacctaa
      301 atacggcatc cgtggcatcc caactttgct cctgttcaag aatggtgaag tagcagctac
      361 caaggtaggc gcactgtcta aaggccagct gaaagaattt ctggacgcca acttagcttt
      421 agttcctcgc ggatccaggc tgaccatccc catctcccag attgcacccg accttatcct
      481 cgctgacctg cctcgcaaca tcatgctgaa caatgacgag ctggagtttg aacaagcacc
      541 cgagttcctg ctaggcgacg gctctttcgg ttcagtttac cgtgctgctt acgagggtga
      601 agaggttgcc gtgaagattt ttaacaagca cacttcgctc cgtctgctgc gtcaggagct
      661 tgtggttctc tgccacctgc accacccttc cctgatctcc ctgctggccg ccggcattag
      721 gcctcgcatg ctagtgatgg aactggccag taagggttca ttggaccgcc tgctgcagca
      781 ggacaaggct agcctgaccc gtacgctcca gcacc
//
