LOCUS       sequence-54300-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Derijard B, Hibi M, Wu IH, Barrett T, Su B, Deng T, Karin M, Davis 
            RJ
  TITLE     JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds 
            and phosphorylates the c-Jun activation domain.
  JOURNAL   Cell. 1994 Mar 25. 76(6):1025-37.
  PUBMED    8137421
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1994 
            Mar 25. 76(6):1025-37."
COMMENT     Sequence Label: pCDNA3 Flag Jnk1a1(apf)
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttcg gcagccaaca tggactataa ggacgatgat gacaaaagca
      121 gaagcaagcg tgacaacaat ttttatagtg tagagattgg agattctaca ttcacagtcc
      181 tgaaacgata tcagaattta aaacctatag gctcaggagc tcaaggaata gtatgcgcag
      241 cttatgatgc cattcttgaa agaaatgttg caatcaagaa gctaagccga ccatttcaga
      301 atcagactca tgccaagcgg gcctacagag agctagttct tatgaaatgt gttaatcaca
      361 aaaatataat tggccttttg aatgttttca caccacagaa atccctagaa gaatttcaag
      421 atgtttacat agtcatggag ctcatggatg caaatctttg ccaagtgatt cagatggagc
      481 tagatcatga aagaatgtcc taccttctct atcagatgct gtgtggaatc aagcaccttc
      541 attctgctgg aattattcat cgggacttaa agcccagtaa tatagtagta aaatctgatt
      601 gcactttgaa gattcttgac ttcggtctgg ccaggactgc aggaacgagt tttatgatgg
      661 cgccttttgt cgtcacacgg tattacagag cacccgaggt catccttggc atgggctaca
      721 aggaaaacgt ggatttatgg tctgtggggt gcattatggg agaaatggtt tgccacaaaa
      781 tcctctttcc aggaagggac tatattgatc agtggaataa agttattgaa cagcttggaa
      841 caccatgtcc tgaattcatg aagaaa
//
