LOCUS       sequence-54425-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Conformation-Selective ATP-Competitive Inhibitors Control Regulatory
            Interactions and Noncatalytic Functions of Mitogen-Activated Protein
            Kinases.
  JOURNAL   Chem Biol. 2014 May 22;21(5):628-35. doi: 
            10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3.
  PUBMED    24704509
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.02.016"; journalName: "Chem Biol"; date: 
            "2014-05-22- 22"; volume: "21"; issue: "5"; pages: "628-35"
COMMENT     Sequence Label: DUSP6
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg gtgtagatct
       61 gggtaccgag aacctgtact tccaatccaa tgccatagat acgctcagac ccgtgccctt
      121 cgcgtcggaa atggcgatca gcaagacggt ggcgtggctc aacgagcagc tggagctggg
      181 caacgagcgg ctgctgctga tggactgccg gccgcaggag ctatacgagt cgtcgcacat
      241 cgagtcggcc atcaacgtgg ccatcccggg catcatgctg cggcgcctgc agaagggtaa
      301 cctgccggtg cgcgcgctct tcacgcgcgg cgaggaccgg gaccgcttca cccggcgctg
      361 tggcaccgac acagtggtgc tctacgacga gagcagcagc gactggaacg agaatacggg
      421 cggcgagtcg gtgctcgggc tgctgctcaa gaagctcaag gacgagggct gccgggcgtt
      481 ctacctggaa ggtggcttca gtaagttcca agccgagttc tccctgcatt gcgagaccaa
      541 tctagacggc tcgtgtagca gcagctcgcc gccgttgcca gtgctggggc tcgggggcct
      601 gcggatcagc tctgactctt cctcggacat cgagtctgac cttgaccgag accccaatag
      661 tgcaacagac tcggatggta gtccgctgtc caacagccag ccttccttcc cagtggagat
      721 cttgcccttc ctctacttgg gctgtgccaa agactccacc aacttggacg tgttggagga
      781 attcggcatc aagtacatct tgaacgtcac ccccaatttg ccgaatctct ttgagaacgc
      841 aggagagttt aaatacaagc aaatccccat ctcggatcac tggagcc
//
